Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Geminin with ab175799 at 1:1000. Cells were fixed with PFA and permeabilized with methanol for 2 minutes. Cells were incubated with the primary anitbody overnight at room temperature. From left to right - Geminin, CyclinB, DAPI, Merge.
Immunohistochemistry (formalin/PFA-fixed paraffin-embedded) analysis of Human linfoid tissue labelling Geminin with ab175799 at 1:1000. Antigen retrieval was by microwave boiling for 7 minutes in 10mM TrisHCl, pH9. Left - 4X, Middle - 10X, Right - 20X.
Immunoprecipitation analysis of U2OS cells labelling Geminin with ab175799. Protein A beads were crosslinked to the serum.
All lanes : Anti-Geminin antibody (ab175799)Lane 1 : Control siLane 2 : Geminin siLane 3 : NIH 3T3 cell lysate at 40 µg