ab84423 staining Geminin in Human tonsil tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in EDTA pH8.0 for 20 minutes at 100°C. Samples were incubated with primary antibody (1/40) for 20 minutes at 25°C. A HPR-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84423, followed by DAB staining.