Anti-Geminin antibody [EPR14637]

• Supplier: Abcam
• Catalog: ab195047
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14637
• Applications: WB ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: Recombinant fragment within Human Geminin aa 100 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: GMNN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Geminin antibody [EPR14637] (ab195047) at 1/500 dilutionLane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with thymidine whole cell lysateLane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with nocodazole whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human triple-negative breast cancer tissue labeling Geminin with ab195047 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human triple-negative breast cancer tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody and ab97051 at 1/500 as secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (Human breast adenocarcinoma cell line) cells labeling Geminin with ab195047 at 1/100 followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500(green). Confocal image showing nuclear staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500  (red).The negative controls are as follows:--ve control 1 - ab195047 at 1/100 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500.