All lanes : Anti-Germinal Center Kinase antibody [EPR14304] (ab184169) at 1/10000 dilutionLane 1 : Namalwa cell lysateLane 2 : Ramos cell lysateLane 3 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma labeling Germinal Center Kinase with ab184169 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of Ramos cells (2% paraformaldehyde-fixed) labeling Germinal Center Kinase with ab184169 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.