Anti-GFAP antibody

• Supplier: Abcam
• Catalog: ab7260
• Prices: $401.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F IHC-F IHC ICC/IF ICC/IF WB IHC-P ICC/IF
• Species Reactivities: Mouse, Rat, Cat, Human, Marmoset, Bovine, Pig, Mammalian
• Antigen: The initial immunization was performed with a preparation of full length human recombinant GFAP expressed in bacteria and highly purified
• Description: Rabbit Polyclonal
• Gene: GFAP
• Conjugate: Unconjugated

Product images

Immunohistochemistical detection of GFAP antibody - Astrocyte Marker (ab7260) on formaldehyde-fixed paraffin-embedded monkey brain sections. Antigen retrieval step: Heat mediated.  Buffer Used: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/2000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti rabbit IgG Conjugated to biotin  (1/200). Marmoset brain: astrocytes are clearly and strongly labelled.See Abreview

ab7260 at 1/10000 dilution staining Mouse cortical astrocytes by Immunocytochemistry. The cells were permeabilized with Triton/HEPES buffer prior to primary application. The antibody was incubated with the cells for 18 hours and then bound antibody was detected with an Alexa Fluor ® 488 conjugated goat anti-rabbit antibody.This image is courtesy of an Abreview submited by Charmaine Noonan.See Abreview

 ab7260 at a 1/5000 dilution staining rat spinal cord tissue sections by IHC-Fr. Rats were transcardially perfused with 4% PFA. The tissue was post fixed 1 hour in 4% PFA and then 30% sucrose for three days. 20µm sections were cryostat cut. The primary antibody was incubated with the tissue sections for 18 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated goat anti-rabbit polyclonal.See Abreview

Western blot of whole rat cerebellum homogenate stained with ab7260 at dilution of 1:100,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~55kDa corresponds to rodent GFAP. A lower band at ~48kDa is derived from the GFAP molecule.

ab7260 staining rat brain tissue sections by IHC-P.  Sections were fixed in formaldehyde and bocoked with a commercialy available blocking agent prior to incubating with ab7260, diluted 1/5000 for 20 hours at 4°C.  A HRP conjugated mouse polyclonal (universal HRP polymer detection) antibody was used as the secondary.See Abreview

All lanes : Anti-GFAP antibody (ab7260) at 1/5000 dilutionLane 1 : Rat thoracotomy, spinal cord homogenateLane 2 : Rat thoracotomy, spinal cord homogenateLane 3 : Rat thoracotomy, spinal cord homogenateLane 4 : Rat thoracotomy sham, spinal cord homogenateLane 5 : Rat thoracotomy sham, spinal cord homogenateLane 6 : Rat nerve transect sham, spinal cord homogenateLane 7 : Rat nerve transect sham, spinal cord homogenateLane 8 : Rat nerve transect, spinal cord homogenateLane 9 : Rat nerve transect, spinal cord homogenateLysates/proteins at 30 µg per lane.SecondaryHRP conjugated goat anti-rabbit at 1/3000 dilutiondeveloped using the ECL techniqueObserved band size : 53 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

IHC - Wholemount of rat retina tissue labelling GFAP (red) with ab7260. Sample was incubated with primary antibody (1/100) for 18 hours at 4°C. A Phycoerythrin-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.See Abreview

ab7260 staining GFAP in mouse eye tissue sections by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C; followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 used at a 1/5000 dilution.The retinal layers are: ganglion cells layer (GCL), inner plexiform layer (IPL), inner nuclear layer (INL), outer plexiform layer (OPL), outer nuclear layer (ONL), and photoreceptor outer segments (ROS). Nuclei were counterstained with DAPI.See Abreview

 ab7260 staining rat pup cortical preps by ICC/IF. The preps were grown for 14 days in culture and plated onto coverslips. The preps were acid/alcohol fixed and blocked prior to incubation with ab7260. Bound antibody was detected using an Alexa Fluor ®488 conjugated goat polyclonal antibody. Nuclei were visualised using DAPI.See Abreview

Product References

The interaction of the atm genotype with inflammation and oxidative stress. - The interaction of the atm genotype with inflammation and oxidative stress.

Yang Y, Hui CW, Li J, Herrup K. PLoS One. 2014 Jan 20;9(1):e85863.

The failure in the stabilization of glioblastoma-derived cell lines: spontaneous - The failure in the stabilization of glioblastoma-derived cell lines: spontaneous

Stoczynska-Fidelus E, Piaskowski S, Bienkowski M, Banaszczyk M, Hulas-Bigoszewska K, Winiecka-Klimek M, Radomiak-Zaluska A, Och W, Borowiec M, Zieba J, Treda C, Rieske P. PLoS One. 2014 Jan 30;9(1):e87136.

Hyaluronic acid-based hydrogel enhances neuronal survival in spinal cord slice - Hyaluronic acid-based hydrogel enhances neuronal survival in spinal cord slice

Schizas N, Rojas R, Kootala S, Andersson B, Pettersson J, Hilborn J, Hailer NP. J Biomater Appl. 2014 Feb;28(6):825-36.

Virally mediated optogenetic excitation and inhibition of pain in freely moving - Virally mediated optogenetic excitation and inhibition of pain in freely moving

Iyer SM, Montgomery KL, Towne C, Lee SY, Ramakrishnan C, Deisseroth K, Delp SL. Nat Biotechnol. 2014 Mar;32(3):274-8.

PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a - PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a

Wang Z, Song F, Li J, Zhang Y, He Y, Yang J, Zhou H, Zhao T, Fu W, Xing P, Wan H, Tian M, Zhang H. Evid Based Complement Alternat Med. 2014;2014:430757.

Prenatal deletion of the RNA-binding protein HuD disrupts postnatal cortical - Prenatal deletion of the RNA-binding protein HuD disrupts postnatal cortical

DeBoer EM, Azevedo R, Vega TA, Brodkin J, Akamatsu W, Okano H, Wagner GC, Rasin MR. J Neurosci. 2014 Mar 5;34(10):3674-86.

PI3Kdelta inhibition reduces TNF secretion and neuroinflammation in a mouse - PI3Kdelta inhibition reduces TNF secretion and neuroinflammation in a mouse

Low PC, Manzanero S, Mohannak N, Narayana VK, Nguyen TH, Kvaskoff D, Brennan FH, Ruitenberg MJ, Gelderblom M, Magnus T, Kim HA, Broughton BR, Sobey CG, Vanhaesebroeck B, Stow JL, Arumugam TV, Meunier FA. Nat Commun. 2014 Mar 14;5:3450.

Sildenafil attenuates vaso-obliteration and neovascularization in a mouse model - Sildenafil attenuates vaso-obliteration and neovascularization in a mouse model

Fawzi AA, Chou JC, Kim GA, Rollins SD, Taylor JM, Farrow KN. Invest Ophthalmol Vis Sci. 2014 Mar 10;55(3):1493-501. doi:

Single-cell western blotting. - Single-cell western blotting.

Hughes AJ, Spelke DP, Xu Z, Kang CC, Schaffer DV, Herr AE. Nat Methods. 2014 Jul;11(7):749-55.

Knock-in reporter mice demonstrate that DNA repair by non-homologous end joining - Knock-in reporter mice demonstrate that DNA repair by non-homologous end joining

Vaidya A, Mao Z, Tian X, Spencer B, Seluanov A, Gorbunova V. PLoS Genet. 2014 Jul 17;10(7):e1004511.