GFAP

Gene Symbol GFAP
Entrez Gene 281189
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description glial fibrillary acidic protein
Other Description -
Swissprots Q28115 Q866S9 Q0P5L4
Accessions AAA51413 AAM19350 AAO18221 DAA18337 Q28115 BC119892 AAI19893 Y08255 CAA69422 XM_010816312 XP_010814614 NM_174065 NP_776490
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subcellular Location Cytoplasm {ECO:0000250}. Note=Associated with intermediate filaments. {ECO:0000250}.

Anti-GFAP (phospho S8) antibody [YC10] - ab115898 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone YC10

Anti-GFAP antibody - ab7260 from Abcam

Prices $401.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistical detection of GFAP antibody - Astrocyte Marker (ab7260) on formaldehyde-fixed paraffin-embedded monkey brain sections. Antigen retrieval step: Heat mediated.  Buffer Used: Citric acid pH6. Permeabilization: None. Blocking step: 1% BSA for 10 mins @ 21°C. Primary antibody incubated at 1/2000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti rabbit IgG Conjugated to biotin  (1/200). Marmoset brain: astrocytes are clearly and strongly labelled.See Abreview

Anti-GFAP antibody - ab53554 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab53554 staining GFAP in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TBS + 0.25% Triton X 100 and blocked with 10% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/1000 in TBS + 0.25% Triton-X 100) for 16 hours at 4°C. A Cy2®-conjugated Donkey anti-goat IgG polyclonal (1/200) was used as the secondary antibody. The image shows GFAP (green) and DAPI (blue) around the dentate gyrus.See Abreview

Anti-GFAP antibody - ab90601 from Abcam

Prices $384.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal
ab90601, at a 1/4000 dilution, staining GFAP in paraffin embedded Human brain tissue (left image) by Immunohistochemistry. Right image shows negative control: 40X objective.

Anti-GFAP antibody - ab116010 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab116010 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab116010 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GFAP antibody - ab138519 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GFAP antibody (ab138519) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Mouse Cortex Tissue LysateLane 4 : Cerebellum Mouse Tissue LysateLane 5 : Cerebellum Rat Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GFAP antibody - ab124455 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
IHC image of GFAP staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124455, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GFAP antibody - ab134436 from Abcam

Prices $378.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.

Anti-GFAP antibody - ab124657 from Abcam

Prices $370.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal

Anti-GFAP antibody - ab118626 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GFAP antibody - ab192713 from Abcam

Prices $370.00
Sizes 50 µl
Clonality Polyclonal

Anti-GFAP antibody [2A5] - ab4648 from Abcam

Prices $400.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A5
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.

Anti-GFAP antibody (HRP) - ab138701 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-GFAP antibody (HRP) (ab138701) at 1/1000 dilution + Mouse Brain lysates (in RIPA buffer) at 35 µgdeveloped using the ECL technique

GFAP Antibody - NBP1-05198 from Novus Biologicals

Prices $299.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Western Blot: GFAP Antibody [NBP1-05198] - Western blot of whole rat cerebellum homogenate stained with NBP1-05198. A prominent band running with an apparent SDS-PAGE molecular weight of ~50kDa corresponds to rodent GFAP. A lower band at ~45kDa is derived from the GFAP molecule.

GFAP Antibody - NB110-93480 from Novus Biologicals

Prices $279.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GFAP Antibody [NB110-93480] - Rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.

GFAP Antibody [PE] - NBP1-05198PE from Novus Biologicals

Prices $319.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal

Glial-Fibrillary-Acidic-Protein-GFAP-Antibody - AN1144 from Abgent, a WuXi AppTec company

Prices $285.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.

Glial-Fibrillary-Acidic-Protein-GFAP-Antibody - AN1143 from Abgent, a WuXi AppTec company

Prices $285.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.

GFAP antibody - 10R-3036 from Fitzgerald

Prices $675.00
Sizes 1 ml
Host Mouse
Clonality Polyclonal
Clone G2584

Anti -GFAP (Glial Fibrillary Acidic Protein) - G2032-27 from USBiological Life Science

Host Rabbit
Clonality Polyclonal

Anti -GFAP (Glial Fibrillary Acidic Protein) - G2032-27A1 from USBiological Life Science

Host Rabbit
Clonality Polyclonal

Anti -GFAP (Glial Fibrillary Acidic Protein) - 214854 from USBiological Life Science

Host Mouse
Clonality Monoclonal

Anti -GFAP (Glial Fibrillary Acidic Protein) - 214855 from USBiological Life Science

Host Mouse
Clonality Monoclonal

Anti -GFAP (Glial Fibrillary Acidic Protein, Astrocyte Marker, FLJ45472, MGC139638) - G2032-26F from USBiological Life Science

Host Rabbit
Clonality Polyclonal

Anti -GFAP (Glial Fibrillary Acidic Protein, Astrocyte) - 215300 from USBiological Life Science

Host Chicken
Clonality Polyclonal
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