Anti-GFAP antibody [2A5]
| Name | Anti-GFAP antibody [2A5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab4648 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2A5 |
| Applications | ELISA ICC/IF IHC-P ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Mouse, Rat, Bovine, Human, Pig, Mammalian |
| Antigen | A preparation of purified pig spinal core GFAP |
| Description | Mouse Monoclonal |
| Gene | GFAP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Rat cortical neurons and glia in mixed tissue culture stained with Chicken polyclonal to MAP2 - ab5392 (green) at 1/30000 and Mouse monoclonal to GFAP - ab4648 (red) at 1/100. Nuclei of all cells are stained with Hoechst dye (blue). Picture taken with a Zeiss 20X objective and documented with a Digital SPOT camera.
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (red).
Rat neuron/glia cultures stained with nmouse monoclonal to GFAP ab 4648 (red).
Rat neuron/glia cultures stained with mouse monoclonal to GFAP ab4648 (green).
Ab4648 staining human substantia nigra. Staining is localised to the cytoplasm.Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![Standard Curve for GFAP; dilution range 1 pg/ml to 1 µg/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 1/5000 and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 µg/ml.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/24073_GFAP-Primary-antibodies-ab4648-7.jpg)
Standard Curve for GFAP; dilution range 1 pg/ml to 1 µg/ml using Capture Antibody Mouse monoclonal [2A5] to GFAP - Astrocyte Marker (ab4648) at 1/5000 and Detector Antibody Rabbit polyclonal to GFAP - Astrocyte Marker (ab48050) at 0.5 µg/ml.
ab4648 staining GFAP - Astrocyte Marker in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody 1/2000 (TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to mouse IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.See Abreview
Product References
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