Anti-Giantin antibody
| Name | Anti-Giantin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24586 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F |
| Species Reactivities | Mouse, Rat, Hamster, Human, Monkey, Zebrafish |
| Antigen | Synthetic peptide, corresponding to N terminal amino acids 1-469 of Human Giantin |
| Description | Rabbit Polyclonal |
| Gene | GOLGB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab24586 at a 1/200 dilution staining mouse C2 myoblasts by ICC/IF. The cells were paraformaldehyde fixed and blocked with 3% BSA and incubated with antibody for 1 hour. Bound antibody was detected using an Alex Fluor ®488 conjugated donkey anti-rabbit IgG (H+L).See Abreview
ab24586 at a 1/500 dilution staining HeLa cells (green) by Immunocytochemistry/Immunofluorescence. Other structures visualized with monoclonal anti-TU27 (red).
ICC/IF image of ab24586 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24586, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab24586 staining Giantin in E10.5 Mouse otic epithelium tissue by Immunohistochemistry (Frozen sections). The sections were PFA-fixed prior to blocking with 5% serum for 1 hour at 37°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor® 568-conjugated Goat anti-Rabbit IgG polyclonal was used as the secondary antibody, diluted 1/500.See Abreview
Anti-Giantin antibody (ab24586) at 1/3000 dilution + A549 whole cell lysate at 80 µgSecondaryHRP conjugated Donkey anti-Rabbit IgG polyclonaldeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Glioma cell proliferation controlled by ERK activity-dependent surface expression - Glioma cell proliferation controlled by ERK activity-dependent surface expression
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Reovirus forms neo-organelles for progeny particle assembly within reorganized - Reovirus forms neo-organelles for progeny particle assembly within reorganized
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ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration. - ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration.
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Parkinson's disease-linked mutations in VPS35 induce dopaminergic - Parkinson's disease-linked mutations in VPS35 induce dopaminergic
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Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte - Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte
Morlino G, Barreiro O, Baixauli F, Robles-Valero J, Gonzalez-Granado JM, Villa-Bellosta R, Cuenca J, Sanchez-Sorzano CO, Veiga E, Martin-Cofreces NB, Sanchez-Madrid F. Mol Cell Biol. 2014 Apr;34(8):1412-26.
Facile bench-top fabrication of enclosed circular microchannels provides 3D - Facile bench-top fabrication of enclosed circular microchannels provides 3D
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RILP regulates vacuolar ATPase through interaction with the V1G1 subunit. - RILP regulates vacuolar ATPase through interaction with the V1G1 subunit.
De Luca M, Cogli L, Progida C, Nisi V, Pascolutti R, Sigismund S, Di Fiore PP, Bucci C. J Cell Sci. 2014 Jun 15;127(Pt 12):2697-708.
The COP II adaptor protein TMED7 is required to initiate and mediate the delivery - The COP II adaptor protein TMED7 is required to initiate and mediate the delivery
Liaunardy-Jopeace A, Bryant CE, Gay NJ. Sci Signal. 2014 Jul 29;7(336):ra70.
A new method to determine in vivo interactomes reveals binding of the Legionella - A new method to determine in vivo interactomes reveals binding of the Legionella
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