GOLGB1

Gene Symbol GOLGB1
Entrez Gene 2804
Alt Symbol GCP, GCP372, GOLIM1
Species Human
Gene Type protein-coding
Description golgin B1
Other Description 372 kDa Golgi complex-associated protein|Golgin subfamily B member 1|giantin|golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1|golgi integral membrane protein 1|golgin B1, golgi integral membrane protein|macrogolgin
Swissprots Q14398 D3DN92 F1T0J2 B2ZZ91 Q14789 E7EP74
Accessions EAW79502 EAW79503 EAW79504 Q14789 AA127633 AB371588 BAG48317 AB593126 BAJ84066 AK094551 AK296903 BAG59460 AK298986 BAG61079 AK300437 BAG62161 BC065301 BF061034 BM475510 BP320001 BX537520 D25542 BAA05025 X75304 CAA53052 XM_005247371 XP_005247428 XM_005247372 XP_005247429 XM_005247373 XP_005247430 XM_006713587 XP_006713650 XM_006713588 XP_006713651 XM_006713589 XP_006713652 XM_006713590 XP_006713653 XM_006713591 XP_006713654 XM_011512699 XP_011511001 XM_011512700 XP_011511002 XR_924125 NM_001256486 NP_001243415 NM_001256487 NP_001243416 NM_001256488 NP_001243417 NM_004487 NP_004478
Function May participate in forming intercisternal cross-bridges of the Golgi complex.
Subcellular Location Golgi apparatus membrane; Single-pass type I membrane protein.

Giantin (N-18) - sc-46993 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Giantin (N-18): sc-46993. Western blot analysis of Giantin expression in HeLa (A), Caki-1 (B) and TT (C) whole cell lysates.

Giantin (K-18) - sc-46992 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Giantin (H-49) - sc-67168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Giantin (H-49): sc-67168. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.

Giantin (2388C3a) - sc-81279 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Giantin (2388C3a): sc-81279 Western Blot analysis of human recombinant Giantin fusion protein.

Anti-Giantin antibody - ab24586 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab24586 at a 1/200 dilution staining mouse C2 myoblasts by ICC/IF. The cells were paraformaldehyde fixed and blocked with 3% BSA and incubated with antibody for 1 hour. Bound antibody was detected using an Alex Fluor ®488 conjugated donkey anti-rabbit IgG (H+L).See Abreview

Anti-Giantin antibody - ab93281 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Giantin antibody (ab93281) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Giantin antibody - ab174655 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Giantin antibody (ab174655) at 0.1 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-Giantin antibody [2388C3a] - ab53542 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2388C3a
Anti-Giantin antibody [2388C3a] (ab53542) + Immunized recombinant protein

Anti-Giantin antibody [9B6] - Golgi Marker - ab37266 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 9B6
The cells were 100% methanol fixed (5 min) and thenincubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500. 

Anti-Giantin antibody (Alexa Fluor® 488) - ab28039 from Abcam

Host Rabbit
Clonality Polyclonal
Formalin fixed HeLa cells stained with Alexa Fluor® 488 Giantin (ab28039) at 1/1000 dilution.

Anti-Giantin antibody - Golgi Marker - ab80864 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab80864 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-GOLGB1 / Giantin Antibody - LS-C66523 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-GOLGB1 / Giantin Antibody (clone 2388C3a) - LS-C184506 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2388C3a

Anti-GOLGB1 / Giantin Antibody - LS-C15337 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-GOLGB1 / Giantin Antibody (clone 6D4) - LS-C197328 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D4
Detection limit for recombinant GST tagged GOLGB1 is approximately 0.03 ng/ml as a capture antibody.

Anti-GOLGB1 / Giantin Antibody (clone 2388C3a) - LS-C342544 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2388C3a
Western blot analysis of immunized recombinant protein, using anti-GOLGB1 monoclonal antibody.

Anti-GOLGB1 / Giantin Antibody (aa425-475) - LS-C290678 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human GOLGB1 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GOLGB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-GOLGB1 / Giantin Antibody (aa1975-2025) - LS-C290680 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human GOLGB1 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GOLGB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-GOLGB1 / Giantin Antibody (aa108-157) IHC-plus™ - LS-B6841 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GOLGB1 / Giantin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6841 concentration 5 ug/ml.

Anti-GOLGB1 / Giantin Antibody (aa950-981) - LS-C290679 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human GOLGB1 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GOLGB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

GOLGB1/Giantin Antibody - AF8159 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, SK‑OV‑3 human ovarian adenocarcinoma cell line, ZR‑75 human breast cancer cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat GOLGB1/Giantin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GOLGB1/Giantin at approximately 370 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GOLGB1/Giantin Antibody - NBP1-91937 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91937] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

GOLGB1/Giantin Antibody - NBP1-91938 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: GOLGB1/Giantin Antibody [NBP1-91938] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

GOLGB1/Giantin Antibody - NBP2-22323 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GOLGB1/Giantin Antibody - NBP2-22322 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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