Anti-Giantin antibody [9B6] - Golgi Marker

• Supplier: Abcam
• Catalog: ab37266
• Prices: $401.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 9B6
• Applications: FC IHC-P ICC/IF ICC/IF
• Species Reactivities: Rat, Human
• Antigen: Full length protein of Rat Giantin
• Description: Mouse Monoclonal
• Gene: GOLGB1
• Conjugate: Unconjugated

Product images

The cells were 100% methanol fixed (5 min) and thenincubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) and (ab37266, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab175652 Alexa Fluor® 405 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. Nuclear Green LCS1 (ab138904) was used to stain the cell nuclei (green) at a dilution of 1/500. 

ICC/IF image of ab37266 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab37266, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

ab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.See Abreview

Overlay histogram showing HeLa cells stained with ab37266 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37266, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Cellular uptake mechanism and intracellular fate of hydrophobically modified - Cellular uptake mechanism and intracellular fate of hydrophobically modified

Jiang L, Li X, Liu L, Zhang Q. Int J Nanomedicine. 2013;8:1825-34.

A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi - A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi

Petrosyan A, Cheng PW. Glycobiology. 2013 Jun;23(6):690-708.

The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates - The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates

Teoule F, Brisac C, Pelletier I, Vidalain PO, Jegouic S, Mirabelli C, Bessaud M, Combelas N, Autret A, Tangy F, Delpeyroux F, Blondel B. J Virol. 2013 Oct;87(20):11031-46.

Opposing effects of bacitracin on human papillomavirus type 16 infection: - Opposing effects of bacitracin on human papillomavirus type 16 infection:

Campos SK, Chapman JA, Deymier MJ, Bronnimann MP, Ozbun MA. J Virol. 2012 Apr;86(8):4169-81.

Global detection of protein kinase D-dependent phosphorylation events in - Global detection of protein kinase D-dependent phosphorylation events in

Franz-Wachtel M, Eisler SA, Krug K, Wahl S, Carpy A, Nordheim A, Pfizenmaier K, Hausser A, Macek B. Mol Cell Proteomics. 2012 May;11(5):160-70.

Growth arrest-specific 1 binds to and controls the maturation and processing of - Growth arrest-specific 1 binds to and controls the maturation and processing of

Chapuis J, Vingtdeux V, Campagne F, Davies P, Marambaud P. Hum Mol Genet. 2011 May 15;20(10):2026-36.

Secretion of glycosylated pro-B-type natriuretic peptide from normal - Secretion of glycosylated pro-B-type natriuretic peptide from normal

Tonne JM, Campbell JM, Cataliotti A, Ohmine S, Thatava T, Sakuma T, Macheret F, Huntley BK, Burnett JC Jr, Ikeda Y. Clin Chem. 2011 Jun;57(6):864-73.

ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites. - ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites.

Sasaki J, Ishikawa K, Arita M, Taniguchi K. EMBO J. 2012 Feb 1;31(3):754-66.

AGAP2 regulates retrograde transport between early endosomes and the TGN. - AGAP2 regulates retrograde transport between early endosomes and the TGN.

Shiba Y, Romer W, Mardones GA, Burgos PV, Lamaze C, Johannes L. J Cell Sci. 2010 Jul 15;123(Pt 14):2381-90.