Anti-Giantin antibody - Golgi Marker

• Supplier: Abcam
• Catalog: ab80864
• Prices: $378.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1250 - 1350 of Human Giantin
• Description: Rabbit Polyclonal
• Gene: GOLGB1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab80864 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

IHC image of Giantin staining in human cervix carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80864, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Product References

Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein - Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein

Mousnier A, Swieboda D, Pinto A, Guedan A, Rogers AV, Walton R, Johnston SL, Solari R. J Virol. 2014 Oct;88(20):11671-85.

14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes. - 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.

Xu Z, Graham K, Foote M, Liang F, Rizkallah R, Hurt M, Wang Y, Wu Y, Zhou Y. J Cell Sci. 2013 Sep 15;126(Pt 18):4173-86.