ab113890, at 5 µg/ml, staining Girdin in Formalin-Fixed, Paraffin-Embedded Human tonsil tissue by immunohistochemistry.
Lane 1 : Anti-Girdin antibody (ab113890) at 1 µg/mlLane 2 : Anti-Girdin antibody (ab113890) at 2 µg/mlLane 1 : Rat brain lysateLane 2 : Rat brain lysate
ICC/IF image of ab113890 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.