All lanes : Anti-GLCNE antibody [EPR15058] (ab184963) at 1/10000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human placenta lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GLCNE with ab184963 at 1/100 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-GLCNE antibody [EPR15058] (ab184963) at 1/20000 dilution + SW480 lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution