All lanes : Anti-GLCNE antibody [EPR15059] - C-terminal (ab189927) at 1/1000 dilutionLane 1 : Human fetal liverLane 2 : Human fetal kidneyLane 3 : Human fetal brainLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-GLCNE antibody [EPR15059] - C-terminal (ab189927) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GLCNE with ab189927 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Western blot analysis of GLCNE in A549 cell lysate immunoprecipitated using ab189927 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.