Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
Anti-Gli1 antibody [EPR4523] (ab134906) at 1/100 dilution (unpurified) + RMS13 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)Lane 1 : RMS13 cell lysateLane 2 : A673 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution