Anti-Glucose Transporter GLUT1 antibody

• Supplier: Abcam
• Catalog: ab652
• Prices: $400.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F ICC/IF Immunomicroscopy IP WB IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1 (Peptide available as ab115830
• Blocking Peptide: Human Glucose Transporter GLUT1 peptide
• Description: Rabbit Polyclonal
• Gene: SLC2A1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Glucose Transporter GLUT1 antibody (ab652) at 1/1000 dilutionLane 1 : 2 week old Mouse brain tissue lysateLane 2 : 2 week old Mouse brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat HRP-conjugate anti-rabbit at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

ab652 at 1/250 staining human renal carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody for 45 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview

ICC/IF image of ab652 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab652, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab652 staining Glucose Transporter GLUT1 in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA was performed for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at dilution at 1/200. DAPI was used to stain the cell nuclei (blue).See Abreview

ab652 staining Glucose Transporter GLUT1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/250 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).See Abreview

Product References

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