SLC2A1

Gene Symbol SLC2A1
Entrez Gene 6513
Alt Symbol DYT17, DYT18, DYT9, EIG12, GLUT, GLUT-1, GLUT1, GLUT1DS, HTLVR, PED
Species Human
Gene Type protein-coding
Description solute carrier family 2 (facilitated glucose transporter), member 1
Other Description glucose transporter type 1, erythrocyte/brain|hepG2 glucose transporter|human T-cell leukemia virus (I and II) receptor|receptor for HTLV-1 and HTLV-2|solute carrier family 2, facilitated glucose transporter member 1
Swissprots B2R620 O75535 Q147X2 P11166 A8K9S6 D3DPX0
Accessions AAB61084 CAI23886 EAX07123 EAX07124 P11166 AB208987 BAD92224 AF070544 AAC28635 AK122999 BAG53842 AK292791 BAF85480 AK293306 BAG56827 AK296736 BAG59323 AK312403 BAG35317 AW137914 AY034633 AAK56795 BC118590 AAI18591 BC121804 AAI21805 BG682043 BI490999 BP314853 BQ948542 DA753077 K03195 AAA52571 NM_006516 NP_006507
Function Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. {ECO:0000269|PubMed:18245775, ECO:0000269|PubMed:19449892}.
Subcellular Location Cell membrane; Multi-pass membrane protein. Melanosome. Note=Localizes primarily at the cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues. {ECO:0000269|PubMed:23219802}.
Top Pathways Adipocytokine signaling pathway, Renal cell carcinoma, Bile secretion, HTLV-I infection, Central carbon metabolism in cancer

Glut1 (D3J3A) Rabbit mAb - 12939 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3J3A
Western blot analysis of extracts from various cell lines using Glut1 (D3J3A) Rabbit mAb.

Glut1 (A-4) - sc-377228 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Glut1 (A-4): sc-377228. Western blot analysis of Glut1 expression in H4 (A) and MDA-MB-231 (B) whole cell lysates.

Glut1 (C-20) - sc-1605 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Glut1 (H-43) - sc-7903 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Glut1 (H-43): sc-7903. Western blot analysis of Glut1 expression in H4 whole cell lysate.

Glut1 (N-20) - sc-1603 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Glucose Transporter GLUT1 antibody - ab652 from Abcam

Prices $400.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glucose Transporter GLUT1 antibody (ab652) at 1/1000 dilutionLane 1 : 2 week old Mouse brain tissue lysateLane 2 : 2 week old Mouse brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryGoat HRP-conjugate anti-rabbit at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-Glucose Transporter GLUT1 antibody - ab15309 from Abcam

Prices $401.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab15309 staining Glucose Transporter GLUT1 (green) in Human red blood cells tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS-T + 1% PBS) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Red - autofluorescence of erythrocytes.See Abreview

Anti-Glucose Transporter GLUT1 antibody - ab32551 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glucose Transporter GLUT1 antibody (ab32551) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-Glucose Transporter GLUT1 antibody - ab54263 from Abcam

Prices $378.00
Sizes 125 µg
Host Sheep
Clonality Polyclonal
ICC/IF image of ab54263 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54263, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96939 donkey anti-sheep Dylight 488 (IgG H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT1 antibody - ab53767 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53767 at a 1/50 dilution.Left image un-treated.Right image treated with peptide.

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - ab115730 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3915
Overlay histogram showing HeLa cells stained with ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 488) - ab195359 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3915
Overlay histogram showing HepG2 cells stained with ab195359 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195359, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Alexa Fluor® 647) - ab195020 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3915
Overlay histogram showing HepG2 cells stained with ab195020 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195020, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in HepG2 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (HRP) - ab195021 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3915
IHC image of Glucose Transporter GLUT1 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195021 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Glucose Transporter GLUT1 antibody, prediluted - ab15310 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab15310 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15310, Neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT1 antibody [SPM498] - ab40084 from Abcam

Prices $401.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone SPM498
ab40084 staining Glucose Transporter GLUT1 in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 20% serum for 20 minutes at room temperature. The sample was incubated with primary antibody (1/200) . A Biotin-conjugated donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-Glucose Transporter GLUT1 antibody [SPM498], prediluted - ab53654 from Abcam

Prices $378.00
Sizes 7 ml
Host Mouse
Clonality Monoclonal
Clone SPM498
ICC/IF image of ab53654 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53654, neat) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-SLC2A1 / GLUT-1 Antibody (C-Terminus, clone SP168) - LS-C210400 from LifeSpan Bioscience

Prices $530.00
Sizes 500 µl
Host Rabbit
Clonality Monoclonal
Clone SP168
Immunohistochemistry of Human Colon Adenocarcinoma stained with anti-Glut-1 antibody

Anti-SLC2A1 / GLUT-1 Antibody - LS-C15376 from LifeSpan Bioscience

Prices $465.00
Sizes 250 µg
Host Sheep
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (C-Terminus) - LS-C15378 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (N-Terminus) - LS-C15377 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (C-Terminus) - LS-C15381 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (C-Terminus) - LS-C15383 from LifeSpan Bioscience

Prices $560.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (C-Terminus) - LS-C15386 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SLC2A1 / GLUT-1 Antibody (aa481-492) - LS-C45655 from LifeSpan Bioscience

Prices $680.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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