Anti-Glucose Transporter GLUT1 antibody [EPR3915]

• Supplier: Abcam
• Catalog: ab115730
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3915
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glucose Transporter GLUT1 aa 450-550
• Blocking Peptide: Glucose Transporter GLUT1 peptide
• Description: Rabbit Monoclonal
• Gene: SLC2A1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000 dilutionLane 1 : Jurkat lysateLane 2 : Mouse brain lysateLane 3 : Human fetal brain lysateLane 4 : 3T3L1 lysateLane 5 : Human fetal liver lysateLane 6 : HepG2 lysateLysates/proteins at 10 µg per lane.

ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.

ab115730 showing positive staining in Normal liver tissue.

ab115730 showing positive staining in Normal breast tissue.

ab115730 showing positive staining in Normal colon tissue.

ab115730 showing positive staining in Kidney carcinoma tissue.

ab115730 showing negative staining in Skeletal muscle tissue.

ab115730 showing positive staining in Urinary bladder transitional carcinoma tissue.

ab115730 showing negative staining in Normal heart tissue.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Instruction of circulating endothelial progenitors in vitro towards specialized - Instruction of circulating endothelial progenitors in vitro towards specialized

Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud PO, Uzan G. PLoS One. 2014 Jan 2;9(1):e84179.

Leptin metabolically licenses T cells for activation to link nutrition and - Leptin metabolically licenses T cells for activation to link nutrition and

Saucillo DC, Gerriets VA, Sheng J, Rathmell JC, Maciver NJ. J Immunol. 2014 Jan 1;192(1):136-44.

Metabolic reprogramming is required for antibody production that is suppressed in - Metabolic reprogramming is required for antibody production that is suppressed in

Caro-Maldonado A, Wang R, Nichols AG, Kuraoka M, Milasta S, Sun LD, Gavin AL, Abel ED, Kelsoe G, Green DR, Rathmell JC. J Immunol. 2014 Apr 15;192(8):3626-36.

Metabolic reprogramming towards aerobic glycolysis correlates with greater - Metabolic reprogramming towards aerobic glycolysis correlates with greater

Cao Y, Rathmell JC, Macintyre AN. PLoS One. 2014 Aug 4;9(8):e104104.

Multiple metabolic alterations exist in mutant PI3K cancers, but only glucose is - Multiple metabolic alterations exist in mutant PI3K cancers, but only glucose is

Foster R, Griffin S, Grooby S, Feltell R, Christopherson C, Chang M, Sninsky J, Kwok S, Torrance C. PLoS One. 2012;7(9):e45061.