Anti-Glucose Transporter GLUT1 antibody, prediluted

• Supplier: Abcam
• Catalog: ab15310
• Prices: $376.00
• Sizes: 7 ml
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P
• Species Reactivities: Human, Rat
• Antigen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal)
• Description: Rabbit Polyclonal
• Gene: SLC2A1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab15310 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15310, Neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab15310 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).