Anti-Glucose Transporter GLUT1 antibody [SPM498]
| Name | Anti-Glucose Transporter GLUT1 antibody [SPM498] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40084 |
| Prices | $401.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | SPM498 |
| Applications | FC ICC/IF ICC/IF IHC-F WB IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic human peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal) |
| Description | Mouse Monoclonal |
| Gene | SLC2A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab40084 staining Glucose Transporter GLUT1 in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 20% serum for 20 minutes at room temperature. The sample was incubated with primary antibody (1/200) . A Biotin-conjugated donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.See Abreview
ab40084 at a 1:200 dilution staining Glucose Transporter GLUT1 in Human esophagous tissue.
![Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084) at 1/500 dilution + Whole cell lysate prepared from human MDA-MB 231 cells at 20 µgSecondaryHRP conjugated sheep polyclonal to mouse IgG at 1/5000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25188_Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-4.jpg)
Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084) at 1/500 dilution + Whole cell lysate prepared from human MDA-MB 231 cells at 20 µgSecondaryHRP conjugated sheep polyclonal to mouse IgG at 1/5000 dilutiondeveloped using the ECL technique
ICC/IF image of ab40084 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)Lane 1 : Cell lysates prepared from NIH3T3 cellsLane 2 : Cell lysates prepared from MDA MB 231 cellsLane 3 : Cell lysates prepared from HeLa cells](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25190_Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-6.jpg)
All lanes : Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)Lane 1 : Cell lysates prepared from NIH3T3 cellsLane 2 : Cell lysates prepared from MDA MB 231 cellsLane 3 : Cell lysates prepared from HeLa cells
ab40084 staining Glucose Transporter GLUT1 in mouse smooth muscle tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/200 im PBS-Tween) at 25°C for 1 hour. An Alexa Fluor®594-conjugated Goat polyclonal to mouse IgG (1/250) was used as secondary antibody. See Abreview
![Overlay histogram showing HeLa cells stained with ab40084 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25192_Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-11.jpg)
Overlay histogram showing HeLa cells stained with ab40084 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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