Anti-Glucose Transporter GLUT1 antibody [SPM498], prediluted

• Supplier: Abcam
• Catalog: ab53654
• Prices: $378.00
• Sizes: 7 ml
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: SPM498
• Applications: ICC/IF ICC/IF IHC-P
• Species Reactivities: Human, Mouse, Rat
• Antigen: Synthetic peptide derived from C-terminal of human Glucose Transporter GLUT1 protein
• Description: Mouse Monoclonal
• Gene: SLC2A1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab53654 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53654, neat) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

ab53654 (pre-diluted) staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemsitry, Formalin-fixed, Parafin embedded tissue.

Product References

Fructose transporter GLUT5 expression in clear renal cell carcinoma. - Fructose transporter GLUT5 expression in clear renal cell carcinoma.

Medina Villaamil V, Aparicio Gallego G, Valbuena Rubira L, Garcia Campelo R, Valladares-Ayerbes M, Grande Pulido E, Victoria Bolos M, Santamarina Cainzos I, Anton Aparicio LM. Oncol Rep. 2011 Feb;25(2):315-23.

The diagnostic utility of p16 FISH and GLUT-1 immunohistochemical analysis in - The diagnostic utility of p16 FISH and GLUT-1 immunohistochemical analysis in

Monaco SE, Shuai Y, Bansal M, Krasinskas AM, Dacic S. Am J Clin Pathol. 2011 Apr;135(4):619-27.