Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody

• Supplier: Abcam
• Catalog: ab12108
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-F IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse Glutamate Receptor 1 (AMPA subtype), phosphorylated at T840
• Blocking Peptide: Mouse Glutamate Receptor 1 (AMPA subtype) (phospho T840) peptide
• Description: Rabbit Polyclonal
• Gene: GRIA1
• Conjugate: Unconjugated

Product images

Lane 1 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 0.3 µg/mlLane 2 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 1 µg/mlLanes 3 - 5 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 3 µg/mlLane 1 : mouse brain extractLane 2 : mouse brain extractLane 3 : mouse brain extractLane 4 : mouse brain extract with Mouse Glutamate Receptor 1 (AMPA subtype) (phospho T840) peptide (ab12399) at 2 µg/mlLane 5 : mouse brain extract with Mouse Glutamate Receptor 1 (AMPA subtype) (unmodified ) peptide (ab12434) at 2 µg/mlPerformed under reducing conditions.

Lanes 1, 4, 7 & 8 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 3 µg/mlLane 2 : Lane 3 : Preimmune mouse IgG antibodyLane 5 : Blot reprobed with GluR1 mouse antibodyLane 6 : Lane 1 : Mouse brain extract that has not been through IP protocolLane 2 : BlankLane 3 : Control IP for mouse IgGLane 4 : Mouse brain extract following IP for GluR1Lane 5 : As aboveLane 6 : BlankLane 7 : Input lane reprobed with GluR1 mouse antibodyLane 8 : IP lane reprobed with GluR1 mouse antibodyPerformed under reducing conditions.

Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) (phospho T840) using antibody ab12108 on PFA perfusion-fixed free floating rat brain sections. Primary Antibody dilution 1/100 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The antibody produced some staining in cytoplasmic neuronal inclusions. The picture shows the staining obtained using the objective X40 at the level of the cerebral cortex. The tissues were perfusion fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.See Abreview

Product References

Involvement of ClC-3 chloride/proton exchangers in controlling glutamatergic - Involvement of ClC-3 chloride/proton exchangers in controlling glutamatergic

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Inhibitory interactions between phosphorylation sites in the C terminus of - Inhibitory interactions between phosphorylation sites in the C terminus of

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Glutamate receptors as targets of protein kinase C in the pathophysiology and - Glutamate receptors as targets of protein kinase C in the pathophysiology and

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NMDA receptor activation dephosphorylates AMPA receptor glutamate receptor 1 - NMDA receptor activation dephosphorylates AMPA receptor glutamate receptor 1

Delgado JY, Coba M, Anderson CN, Thompson KR, Gray EE, Heusner CL, Martin KC, Grant SG, O'Dell TJ. J Neurosci. 2007 Nov 28;27(48):13210-21.