Anti-Glutamate Receptor 1 (AMPA subtype) antibody

• Supplier: Abcam
• Catalog: ab31232
• Prices: $394.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-F IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human, Marmoset, Monkey
• Antigen: Synthetic peptide derived from within residues 850 to the C-terminus of Human Glutamate Receptor 1 (AMPA subtype)
• Description: Rabbit Polyclonal
• Gene: GRIA1
• Conjugate: Unconjugated

Product images

Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype)  with ab31232 on formaldehyde-fixed paraffin-embedded rat cerebellum sections. Antigen retrieval step: heat mediated  in citric acid pH6. Blocking step: 1% BSA  for 10 mins @ rt°C.  Primary antibody was incubated at 1/200 for 2 hours @ room temp. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows localisation of this protein apparently exclusive to the molecular layer of the cerebellum upper image (low power). In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted.See Abreview

Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) at 1 µg/ml + Mouse Brain Tissue Lysate at 20 µgSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

ab31232 staining Glutamate Receptor 1 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. See Abreview

Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) with ab31232 on  formaldehyde-fixed paraffin-embedded mouse brain sections. Antigen retrieval step: heat mediated in Citric acid pH6 buffer.Blocking step: 1%BSA for 10 mins @ rt°C. Primary antibody ab31232 incubated at 1/100 for 2 hours. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows immunostaining in cell bodies and processes of a subset of neurones in the mouse cortex (upper image) and also in the dendritic fields of the molecular layer of the mouse cerebellum (lower image). Purkinje cells of the cerebellum are negative but their primary processes are clearly delineated by a punctate membrane-region positivity. What appears to be small cells in close contact with the Purkinje cell bodies are strongly positive (perhaps these are basket cells?).

IHC image of Glutamate Receptor 1 (AMPA subtype) staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31232, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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