Anti-Glutaminase antibody
| Name | Anti-Glutaminase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab93434 |
| Prices | $398.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC WB ICC/IF ICC/IF IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Glutaminase aa 516-545 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | GLS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Glutaminase antibody (ab93434) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Human brain tissue lysateLane 5 : Mouse brain tissue lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Anti-Glutaminase antibody (ab93434) at 1/1000 dilution + Rat kidney tissue lyate at 35 µgSecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Anti-Glutaminase antibody (ab93434) at 1/1000 dilution + mouse liver tissue lysate at 35 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
ICC/IF image of ab93434 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Glutaminase (green) with ab93434. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counter stained with DAPI (blue).
IHC image of ab93434 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93434, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling Glutaminase with ab93434. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody.
Flow cytometry analysis of HepG2 cells labelling Glutaminase (green) with ab93434 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Product References
Expression of glutaminase is upregulated in colorectal cancer and of clinical - Expression of glutaminase is upregulated in colorectal cancer and of clinical
Huang F, Zhang Q, Ma H, Lv Q, Zhang T. Int J Clin Exp Pathol. 2014 Feb 15;7(3):1093-100. eCollection 2014.
Systematic design and functional analysis of artificial microRNAs. - Systematic design and functional analysis of artificial microRNAs.
Arroyo JD, Gallichotte EN, Tewari M. Nucleic Acids Res. 2014 May;42(9):6064-77.
Molecular basis for the differential use of glucose and glutamine in cell - Molecular basis for the differential use of glucose and glutamine in cell
Colombo SL, Palacios-Callender M, Frakich N, Carcamo S, Kovacs I, Tudzarova S, Moncada S. Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21069-74. doi: