GLS

Gene Symbol GLS
Entrez Gene 2744
Alt Symbol AAD20, GAC, GAM, GLS1, KGA
Species Human
Gene Type protein-coding
Description glutaminase
Other Description K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase kidney isoform, mitochondrial|glutaminase, phosphate-activated
Swissprots Q9UL07 Q9UL05 Q9UL06 O94925 Q9UN40
Accessions AAY14767 AAY24182 AAY24320 EAX10856 EAX10857 EAX10858 EAX10859 EAX10860 O94925 AB020645 BAA74861 AF097492 AAF00088 AF097493 AAF00089 AF097494 AF097495 AAF00090 AF158555 AAD47056 AF223943 AAF33825 AF279697 AAG17700 AF327434 AAG47842 AJ431620 AK001220 BAG50870 AK092974 AK123493 AK289747 BAF82436 AK294927 BAH11927 AK298266 BAH12745 BC038507 AAH38507 BM148085 BX641148 CR749593 CAH18392 Y08264 XM_005246467 XP_005246524 XM_006712435 XP_006712498 XR_241306 XR_922905 NM_001256310 NP_001243239 NM_014905 NP_055720
Function Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Subcellular Location Isoform 1: Cytoplasm, cytosol.
Tissue Specificity Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. {ECO:0000269|PubMed:11015561}.
Top Pathways Central carbon metabolism in cancer, MicroRNAs in cancer, Glutamatergic synapse, GABAergic synapse, Proximal tubule bicarbonate reclamation

KGA (H-45) - sc-366447 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

KGA (T-16) - sc-26646 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
KGA (T-16): sc-26646. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.

KGA (IJ-2) - sc-100533 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
KGA (IJ-2): sc-100533. Western blot analysis of KGA expression in HeLa nuclear extract.

Anti-Glutaminase antibody - ab93434 from Abcam

Prices $398.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glutaminase antibody (ab93434) at 1/1000 dilutionLane 1 : 293 cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLane 4 : Human brain tissue lysateLane 5 : Mouse brain tissue lysateLysates/proteins at 35 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

Anti-Glutaminase antibody - ab60709 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Glutaminase antibody (ab60709) + HeLa whole cell lysate at 50 µgSecondaryGoat Anti-Mouse IgG HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-Glutaminase antibody - ab131554 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab131554 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131554 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glutaminase antibody - ab98967 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Glutaminase antibody (ab98967) at 1 µg/ml + ACHN cell lysate at 10 µg

Anti-Glutaminase antibody [EP7212] - ab156876 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7212
ab156876 showing +ve staining in Human normal tonsil.

Anti-Glutaminase antibody [EP7212] (Alexa Fluor® 488) - ab200406 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7212
ab200406 staining Glutaminase in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200406 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Glutaminase antibody [EP7212] (Alexa Fluor® 647) - ab200407 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7212
ab200407 staining Glutaminase in A673 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200407 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GLS / Glutaminase Antibody (aa580-670, clone 10C382, Azide-free) - LS-C183654 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10C382

Anti-GLS / Glutaminase Antibody (C-Terminus, Biotin) - LS-C244464 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus, AP) - LS-C244463 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus, APC) - LS-C244466 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus, PE) - LS-C244467 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus, FITC) - LS-C244465 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus, Azide-free, HRP) - LS-C247595 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (HRP) - LS-C318883 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody - LS-C285796 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (FITC) - LS-C317372 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus) - LS-C330455 from LifeSpan Bioscience

Prices $425.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GLS / Glutaminase Antibody (C-Terminus) - LS-C111602 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
GLS antibody LS-C111602 Western blot of ACHN lysate.

Anti-GLS / Glutaminase Antibody - LS-C332871 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using GLS antibody.

Anti-GLS / Glutaminase Antibody (clone 5C4) - LS-C197311 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5C4
Immunofluorescence of monoclonal antibody to GLS on HeLa cell. [antibody concentration 10 ug/ml]

Glutaminase Antibody - NBP2-29940 from Novus Biologicals

Prices $79.00, $329.00
Sizes 25 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Glutaminase Antibody [NBP2-29940] - Western blot analysis of lysates from human brain tissue and Hela cell line (from left to right), using (C-term)(Cat. # NBP2-29940). The antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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