All lanes : Anti-Glutamine Synthetase antibody [1F4] (ab118059) at 1/2000 dilutionLane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY Glutamine Synthetase Lysates/proteins at 5 µg per lane.
All lanes : Anti-Glutamine Synthetase antibody [1F4] (ab118059) at 1/2000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab118059 at 1/50 dilution staining Glutamine Synthetase in Paraffin-embedded Human Breast tissue by Immunohistochemistry.
ab118059 at 1/50 dilution staining Glutamine Synthetase in Paraffin-embedded Human Ovary tissue by Immunohistochemistry.
ab118059 at 1/50 dilution staining Glutamine Synthetase in Paraffin-embedded Human Endometrium tissue by Immunohistochemistry.
ab118059 at 1/100 dilution staining Glutamine Synthetase in COS7 cells transiently transfected by pCMV6-ENTRY Glutamine Synthetase by Immunofluorescence.
ab118059 at 1/100 dilution staining Glutamine Synthetase in HepG2 cells by Immunofluorescence.
ab118059 at 1/100 dilution staining Glutamine Synthetase in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab118059 at 1/100 dilution staining Glutamine Synthetase in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).