All lanes : Anti-Glutamine Synthetase antibody [EPR13021] (ab178422) at 1/1000 dilutionLane 1 : Human fetal liver lysatesLane 2 : HeLa cell lysatesLane 3 : HT29 cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of HT29 cells labeling Glutamine Synthetase with ab178422 at 1/250 dilution (red). DAPI nuclear staining (blue).
Flow Cytometric analysis of permeabilized HeLa cells labeling Glutamine Synthetase with ab178422 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).