All lanes : Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human glioma tissue labeling Glutamine Synthetase with ab176562 at a 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human liver tissue labeling Glutamine Synthetase with ab176562 at a 1/100 dilution.
Flow cytometry analysis of permeabilized HeLa cells labeling Glutamine Synthetase (red) with ab176562 at a 1/10 dilution, or negative control rabbit IgG (green).
Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing Glutamine Synthetase, with ab176562, diluted in 1% BSA, at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.