All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilution + Human glioma lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-Glutamine Synthetase antibody [EPR16661] (ab197024) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse spleen lysateLane 3 : NIH/3T3 (Mouse embryo fibroblast cells) lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Glutamine Synthetase with ab197024 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.