Anti-Glutaredoxin 2 antibody - C-terminal

• Supplier: Abcam
• Catalog: ab85267
• Prices: $370.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB ELISA
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide selected from the C terminal region of human Glutaredoxin 2 conjugated to KLH (NP_932066)
• Description: Rabbit Polyclonal
• Gene: GLRX2
• Conjugate: Unconjugated

Product images

Anti-Glutaredoxin 2 antibody - C-terminal (ab85267) at 1/50 dilution + mouse cerebellum tissue lysates at 35 µg

ICC/IF image of ab85267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Glutathionylation state of uncoupling protein-2 and the control of - Glutathionylation state of uncoupling protein-2 and the control of

Mailloux RJ, Fu A, Robson-Doucette C, Allister EM, Wheeler MB, Screaton R, Harper ME. J Biol Chem. 2012 Nov 16;287(47):39673-85.

Crucial yet divergent roles of mitochondrial redox state in skeletal muscle vs. - Crucial yet divergent roles of mitochondrial redox state in skeletal muscle vs.

Mailloux RJ, Adjeitey CN, Xuan JY, Harper ME. FASEB J. 2012 Jan;26(1):363-75.