Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling glutathione S transferase Omega 1 with ab174253 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.
Anti-glutathione S transferase Omega 1 antibody (ab174253) at 1/50 dilution + NCI-H460 cell line lysates at 35 µg
All lanes : Anti-glutathione S transferase Omega 1 antibody (ab174253) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysatesLane 2 : 293 cell lysates transiently transfected with the glutathione S transferase Omega 1 geneLysates/proteins at 2 µg per lane.
Flow cytometric analysis of NCI-H292 cells labeling glutathione S transferase Omega 1 with ab174253 at 1/10 (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.