Anti-Glypican 3 antibody
| Name | Anti-Glypican 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66596 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Pig, Chimpanzee |
| Antigen | A region within synthetic peptide: STIHDSIQYV QKNAGKLTTT IGKLCAHSQQ RQYRSAYYPE DLFIDKKVLK, corresponding to internal sequence amino acids 325-374 of Human Glypican 3 Run BLAST with Run BLAST with |
| Blocking Peptide | Human Glypican 3 peptide |
| Description | Rabbit Polyclonal |
| Gene | GPC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Glypican 3 antibody (ab66596) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab66596 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66596, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab66596 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66596, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
GPC3 expression in mouse ovarian cancer induces GPC3specific T cell-mediated - GPC3 expression in mouse ovarian cancer induces GPC3specific T cell-mediated
Luo C, Shibata K, Suzuki S, Kajiyama H, Senga T, Koya Y, Daimon M, Yamashita M, Kikkawa F. Oncol Rep. 2014 Sep;32(3):913-21.
An inducible hepatocellular carcinoma model for preclinical evaluation of - An inducible hepatocellular carcinoma model for preclinical evaluation of
Runge A, Hu J, Wieland M, Bergeest JP, Mogler C, Neumann A, Geraud C, Arnold B, Rohr K, Komljenovic D, Schirmacher P, Goerdt S, Augustin HG. Cancer Res. 2014 Aug 1;74(15):4157-69.
Characterization of non-alcoholic steatohepatitis-derived hepatocellular - Characterization of non-alcoholic steatohepatitis-derived hepatocellular
Takakura K, Koido S, Fujii M, Hashiguchi T, Shibazaki Y, Yoneyama H, Katagi H, Kajihara M, Misawa T, Homma S, Ohkusa T, Tajiri H. Anticancer Res. 2014 Sep;34(9):4849-55.