GPC3

Gene Symbol GPC3
Entrez Gene 2719
Alt Symbol DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1
Species Human
Gene Type protein-coding
Description glypican 3
Other Description glypican proteoglycan 3|glypican-3|heparan sulphate proteoglycan|intestinal protein OCI-5|secreted glypican-3
Swissprots Q2L880 C9JLE3 Q2L882 G3V1R0 P51654
Accessions AAA98131 AAB87062 CAJ44931 CAO00500 CBF57584 EAX11769 EAX11770 EAX11771 P51654 AK222761 BAD96481 AK222766 BAD96486 AK300168 BAG61950 AK310196 AK310689 BC035972 AAH35972 BX329946 DB107651 DQ349136 ABC72125 DQ349137 ABC72126 DQ349138 ABC72127 DQ893658 ABM84584 DQ895822 ABM86748 JQ943686 AFM30911 L47125 AAA98132 L47176 AAB58806 U50410 AAA93471 Z37987 CAA86069 NM_001164617 NP_001158089 NM_001164618 NP_001158090 NM_001164619 NP_001158091 NM_004484 NP_004475
Function Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. {ECO:0000269|PubMed:17549790}.
Subcellular Location Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}; Extracellular side {ECO:0000250}.
Tissue Specificity Highly expressed in lung, liver and kidney.
Top Pathways Proteoglycans in cancer

glypican-3 (1G12) - sc-65443 from Santa Cruz Biotechnology

Prices $279.00
Sizes 15 µg/100 µl
Host Mouse
Clonality Monoclonal
glypican-3 (1G12): sc-65443. Western blot analysis of glypican-3 expression in 293T whole cell lysate.

glypican-3 (F-3) - sc-390587 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
glypican-3 (F-3): sc-390587. Western blot analysis of glypican-3 expression in COLO320DM (A), SW480 (B) and HTC 116 (C) whole cell lysates.

glypican-3 (C-17) - sc-34095 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-3 (C-17): sc-34095. Western blot analysis of glypican-3 expression in IMR-32 (A) and SH-SY5Y (B) whole cell lysates.

glypican-3 (N-15) - sc-17611 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-3 (N-15): sc-17611. Western blot analysis of glypican-3 expression in NCl-H292 whole cell lysate (A) and human stomach tissue extract (B).

glypican-3 (C-20) - sc-17613 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-3 (K-20): sc-17613. Western blot analysis of glypican-3 expression in HEK293 whole cell lysate.

glypican-3 (G-16) - sc-10456 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-3 (G-16): sc-10456. Western blot analysis of glypican-3 expression in PC-12 whole cell lysate.

glypican-3 (F-18) - sc-34096 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

glypican-3 (H-162) - sc-11395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
glypican-3 (H-162): sc-11395. Western blot analysis of human recombinant glypican-3 fusion protein.

glypican-3 (H-60) - sc-292920 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
glypican-3 (H-60): sc-292920. Western blot analysis of glypican-3 expression in Jurkat (A) and SH-SY5Y (B) whole cell lysates.

glypican-3 (W-18) - sc-10455 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-3 (W-18): sc-10455. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

glypican-3 (H-10) - sc-377266 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
glypican-3 (H-10): sc-377266. Western blot analysis of glypican-3 expression in COLO 320DM whole cell lysate.

Anti-Glypican 3 antibody - ab66596 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Glypican 3 antibody (ab66596) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Glypican 3 antibody - ab101478 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glypican 3 antibody (ab101478) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Glypican 3 antibody - ab186872 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Glypican 3 antibody (ab186872) at 1/500 dilutionLane 1 : BT474 cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : 22RV-1 cell lysateLane 5 : Mouse kidney lysateLane 6 : Mouse testis cell lysate

Anti-Glypican 3 antibody - ab81263 from Abcam

Prices $376.00
Sizes 250 µg
Host Sheep
Clonality Polyclonal
ab81263, at 10ug/ml, staining human hepatocellular carcinoma tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary antibody, HRP conjugated mouse antisheep used at 1:20 dilution. Sections heated for 10 minutes with 10 mM sodium citrate.

Anti-Glypican 3 antibody - ab60125 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorecence staining of ab60125 on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1/50. Original magnification 1:400.

Anti-Glypican 3 antibody - ab93880 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ab993880 at 1/50 dilution staining Glypican 3 in Human liver cancer by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-Glypican 3 antibody - ab190641 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Glypican 3 antibody (ab190641) at 1/500 dilution + HepG2 cell extract at 30 µg

Anti-Glypican 3 antibody [9C2] - ab129381 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9C2
ab129381 at 1/200 dilution (green) or negative control (red) staining Glypican 3 in Jurkat cells by Flow Cytometry.

Anti-Glypican 3 antibody [EPR10641] - ab174851 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10641
Flow cytometric analysis of permeabilized HepG2 cells labeling Glypican 3 using ab174851 at 1/10 dilution (red)  or a rabbit IgG negative (green).

Anti-Glypican 3 antibody [EPR5547] - ab124829 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5547
All lanes : Anti-Glypican 3 antibody [EPR5547] (ab124829) at 1/10000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Glypican 3 antibody [EPR5547] (Alexa Fluor® 488) - ab199736 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5547
ab199736 staining Glypican 3 in A549 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199736 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Glypican 3 antibody [EPR5547] (Alexa Fluor® 647) - ab199813 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5547
ab199813 staining Glypican 3 in A549 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199813 at 1/50 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Glypican 3 antibody [EPR5547] (HRP) - ab199470 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5547
All lanes : Anti-Glypican 3 antibody [EPR5547] (HRP) (ab199470) at 1/5000 dilutionLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Glypican 3 antibody [SP86] - ab95363 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP86
Flow cytometric analysis of rabbit anti-Glypican 3 (SP86) antibody ab98363 (1/100) in HEPG2 cellls (green) compared to negative control of rabbit IgG (blue).
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