Anti-Glypican 4 antibody [AT4A3]
| Name | Anti-Glypican 4 antibody [AT4A3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab100843 |
| Prices | $384.00 |
| Sizes | 50 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AT4A3 |
| Applications | WB ELISA ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment purified from E |
| Description | Mouse Monoclonal |
| Gene | GPC4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HEK293 cells stained with ab100843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab100843, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25921_ab100843-3-ab100843FC.jpg)
Overlay histogram showing HEK293 cells stained with ab100843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab100843, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-Glypican 4 antibody [AT4A3] (ab100843) at 1/1000 dilution + Mouse Kidney Lysates at 35 µgSecondarygoat anti-mouse conjugated to HRP developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/25922_Glypican-4-Primary-antibodies-ab100843-1.jpg)
Anti-Glypican 4 antibody [AT4A3] (ab100843) at 1/1000 dilution + Mouse Kidney Lysates at 35 µgSecondarygoat anti-mouse conjugated to HRP developed using the ECL technique
Immunofluorescence of Human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and ab100843 (1/500) with Texas Red (Red).