GPC4

Gene Symbol GPC4
Entrez Gene 2239
Alt Symbol K-glypican
Species Human
Gene Type protein-coding
Description glypican 4
Other Description dJ900E8.1 (glypican 4)|glypican proteoglycan 4|glypican-4
Swissprots Q96L43 B2R6J7 O75487 Q9UJN1 Q9NU08 B4E2C0 Q6ZMA6 Q9UPD9
Accessions AAF22364 EAX11772 O75487 AF030186 AAC69991 AF064826 AAC31899 AF088063 AK304207 BAG65082 AK312605 BAG35494 AY052833 AAL11018 AY358507 AAQ88871 BC017166 AAH17166 DQ891809 ABM82735 DQ894992 ABM85918 NM_001448 NP_001439
Function Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity). {ECO:0000250}.
Subcellular Location Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}; Extracellular side {ECO:0000250}.
Top Pathways Wnt signaling pathway

glypican-4 (N-16) - sc-247026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

glypican-4 (S-17) - sc-247028 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
glypican-4 (S-17): sc-247028. Western blot analysis of glypican-4 expression in Jurkat (A), K-562 (B), RAW 264.7 (C) and KNRK (D) whole cell lysates and mouse kidney tissue extract (E).

Anti-Glypican 4 antibody - ab52509 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab52509 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52509, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glypican 4 antibody - ab174375 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Glypican 4 antibody (ab174375) at 1/50 dilution + 293 cell line lysate at 35 µg

Anti-Glypican 4 antibody - ab116704 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab116704, at 5-10 µg/ml, staining Glypican 4 in Formalin-fixed, Paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Glypican 4 antibody [AT4A3] - ab100843 from Abcam

Prices $384.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone AT4A3
Overlay histogram showing HEK293 cells stained with ab100843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab100843, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Glypican 4 antibody [EPR11271] - ab168364 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11271
All lanes : Anti-Glypican 4 antibody [EPR11271] (ab168364) at 1/1000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : 293T cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-GPC4 / Glypican 4 Antibody (clone AT4A3) - LS-C113046 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone AT4A3

Anti-GPC4 / Glypican 4 Antibody (aa54-66) - LS-C123636 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa88-101) - LS-C123637 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa220-233) - LS-C123638 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa367-382) - LS-C123639 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa509-520) - LS-C123640 from LifeSpan Bioscience

Prices $405.00
Sizes 20 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (Internal) - LS-C210119 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa222-468, Biotin) - LS-C297323 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa261-527, Biotin) - LS-C300048 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa220-426, Biotin) - LS-C298322 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa261-527, FITC) - LS-C301875 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (FITC) - LS-C318111 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa220-426, FITC) - LS-C301876 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa222-468, FITC) - LS-C304358 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (C-Terminus) - LS-C187321 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HT1080. GPC4 antibody dilution 1.0 ug/ml.

Anti-GPC4 / Glypican 4 Antibody (Biotin) - LS-C318112 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody - LS-C319020 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPC4 / Glypican 4 Antibody (aa261-527) - LS-C294308 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant GPC4 / Glypican 4.
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