Anti-GLYR1 antibody
| Name | Anti-GLYR1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab124615 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Chicken, Bovine, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GLYR1 |
| Description | Rabbit Polyclonal |
| Gene | GLYR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab124615 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab124615 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab124615 (1/400) staining GLYR1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview
All lanes : Anti-GLYR1 antibody (ab124615) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 5 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 7 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.