GLYR1

Gene Symbol GLYR1
Entrez Gene 84656
Alt Symbol BM045, HIBDL, N-PAC, NP60
Species Human
Gene Type protein-coding
Description glyoxylate reductase 1 homolog (Arabidopsis)
Other Description 3-hydroxyisobutyrate dehydrogenase-like protein|cytokine-like nuclear factor n-pac|nuclear protein 60 kDa|nuclear protein 60kDa|nuclear protein NP60|nuclear protein of 60 kDa|putative oxidoreductase GLYR1
Swissprots Q6P1Q2 B4DL47 Q9BXK2 C9JJ60 Q49A26 C9JJ40 Q6V3W7 Q5U632 Q9BTI1
Accessions EAW85251 EAW85252 EAW85253 EAW85254 EAW85255 EAW85256 EAW85257 Q49A26 AF217521 AAF67632 AF244907 AAQ14242 AF326966 AAK15524 AK296842 BAG59409 AK307834 AY352585 AAQ57265 BC003693 AAH03693 BC022809 BC032855 AAH32855 BC047223 AAH47223 BC048291 BC063039 BC064940 AAH64940 BC071698 BC073868 CB529066 CK430984 CR933687 DB492328 XM_005255637 XP_005255694 XM_005255638 XP_005255695 XM_005255639 XP_005255696 XM_005255640 XP_005255697 XM_011522716 XP_011521018 XM_011522717 XP_011521019 XM_011522718 XP_011521020 XR_243321 XR_932954 NM_032569 NP_115958
Function May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3). {ECO:0000269|PubMed:16352664, ECO:0000269|PubMed:20850016}.
Subcellular Location Nucleus {ECO:0000269|PubMed:16352664}.

NP60 (D-10) - sc-390601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NP60 (D-10): sc-390601. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

NP60 (C-12) - sc-136764 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NP60 (C-12): sc-136764. Western blot analysis of NP60 expression in HeLa whole cell lysate.

Anti-GLYR1 antibody - ab124615 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab124615 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab124615 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GLYR1 antibody - ab69277 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-GLYR1 antibody (ab69277) at 1/500 dilutionLane 1 : GLYR1 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-GLYR1 antibody [EPR10076(B)] - ab154838 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10076(B)
All lanes : Anti-GLYR1 antibody [EPR10076(B)] (ab154838) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : HUVEC cell lysateLysates/proteins at 10 µg per lane.

Anti-GLYR1 antibody [EPR10077(B)] - ab167155 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10077(B)
ab167155 showing +ve staining in Mouse kidney.

Anti-GLYR1 Antibody (N-Terminus) - LS-C345959 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of GLYR1 Antibody with HT1080 Whole Cell lysate.

N-PAC Antibody - 21580002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: N-PAC Antibody [21580002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 61, 59 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

N-PAC Antibody - NBP2-38618 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: N-PAC Antibody [NBP2-38618] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

N-PAC Antibody - NBP2-38576 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: N-PAC Antibody [NBP2-38576] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

N-PAC Antibody - H00084656-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: N-PAC Antibody [H00084656-B01] - analysis of N-PAC expression in transfected 293T cell line by N-PAC MaxPab polyclonal antibody (B01). Lane1: N-PAC transfected lysate (52.50 KDa). Lane2: Non-transfected lysate.

N-PAC Antibody - H00084656-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: N-PAC Antibody [H00084656-B01P] - Analysis of N-PAC expression in transfected 293T cell line by N-PAC polyclonal antibody. Lane1:N-PAC transfected lysate(53.24 KDa). Lane2:Non-transfected lysate.

GLYR1/NP60 Polyclonal Antibody - bs-13451R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-13451R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-13451R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-13451R-A555 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-13451R-A647 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, Cy3 Conjugated - bs-13451R-Cy3 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, Cy5 Conjugated - bs-13451R-Cy5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, Cy5.5 Conjugated - bs-13451R-Cy5.5 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, Cy7 Conjugated - bs-13451R-Cy7 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, FITC Conjugated - bs-13451R-FITC from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, Biotin Conjugated - bs-13451R-Biotin from Bioss

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

GLYR1/NP60 Polyclonal Antibody, HRP Conjugated - bs-13451R-HRP from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GLYR1 (533-545) antibody produced in rabbit - SAB1103092 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human GLYR1 was separated on SDS-PAGE, blotted with Anti-GLYR1 (533-545) (<B>Cat. No. SAB1103092</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + GLYR1 immunizing peptide (human, 533-545)
Search more