All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilutionLane 1 : HEK293 (Human embryonic kidney) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse kidney lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat kidney lysateLane 6 : C6 (Rat glial tumor cells) whole cell lysateLane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution