Anti-GOLPH2 antibody
| Name | Anti-GOLPH2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22209 |
| Prices | $359.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide: KNEFQGELEK QREQL , corresponding to amino acids 67-81 of Human GOLPH2 |
| Description | Rabbit Polyclonal |
| Gene | GOLM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab22209 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22209, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab22209 (4µg/ml) staining gGOLPH2n human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic within the colonic epithelium.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-GOLPH2 antibody (ab22209) at 1 µg/mlLane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 2 : Prostate Tumor Lysate Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Golgi phosphoprotein 2 (GOLPH2) expression in liver tumors and its value as a - Golgi phosphoprotein 2 (GOLPH2) expression in liver tumors and its value as a
Riener MO, Stenner F, Liewen H, Soll C, Breitenstein S, Pestalozzi BC, Samaras P, Probst-Hensch N, Hellerbrand C, Mullhaupt B, Clavien PA, Bahra M, Neuhaus P, Wild P, Fritzsche F, Moch H, Jochum W, Kristiansen G. Hepatology. 2009 May;49(5):1602-9.
GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: - GOLPH2 protein expression as a novel tissue biomarker for prostate cancer:
Kristiansen G, Fritzsche FR, Wassermann K, Jager C, Tolls A, Lein M, Stephan C, Jung K, Pilarsky C, Dietel M, Moch H. Br J Cancer. 2008 Sep 16;99(6):939-48.