GOLM1

Gene Symbol GOLM1
Entrez Gene 51280
Alt Symbol C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3
Species Human
Gene Type protein-coding
Description golgi membrane protein 1
Other Description Golgi membrane protein 1|epididymis luminal protein 46|golgi membrane protein GP73|golgi phosphoprotein 2|golgi protein, 73-kD
Swissprots Q8NBJ4 Q9NRB9 Q6IAF4
Accessions AAM87790 AAM87857 CAD32484 CAI72214 CAK32495 EAW62705 EAW62706 Q8NBJ4 AF236056 AAF44663 AI929234 AJ420432 AK027717 BAG51371 AK074188 BAB85012 AK075542 BAC11685 AK075557 BAG52167 AK092943 AL533767 AY358593 AAQ88956 BC001740 AAH01740 BQ216766 CA446701 CR457201 CAG33482 DA116177 EU794616 ACJ13670 HQ447901 ADQ32385 NM_016548 NP_057632 NM_177937 NP_808800 NM_001099268
Function Unknown. Cellular response protein to viral infection.
Subcellular Location Golgi apparatus, cis-Golgi network membrane {ECO:0000269|PubMed:10831838, ECO:0000269|PubMed:12191016}; Single-pass type II membrane protein {ECO:0000269|PubMed:10831838, ECO:0000269|PubMed:12191016}. Note=Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
Tissue Specificity Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. {ECO:0000269|PubMed:10831838, ECO:0000269|PubMed:12029628}.

GP73 (F-2) - sc-365817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GP73 (F-2): sc-365817. Western blot analysis of GP73 expression in HeLa whole cell lysate.

GP73 (F-12) - sc-393372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GP73 (F-12): sc-393372. Western blot analysis of GP73 expression in HeLa whole cell lysate.

GP73 (C-14) - sc-48010 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GP73 (C-14): sc-48010. Western blot analysis of GP73 expression in HeLa (A) and SW480 (B) whole cell lysates.

GP73 (N-19) - sc-48014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GP73 (N-19): sc-48014. Western blot analysis of GP73 expression in c4 whole cell lysate (A) and mouse prostate tissue extract (B).

GP73 (H-114) - sc-134509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GP73 (H-114): sc-134509. Western blot analysis of GP73 expression in mouse brain tissue extract.

Anti-GOLPH2 antibody - ab92612 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of GOLPH2 in rat brain tissue with ab92612 at 20 ug/mL.

Anti-GOLPH2 antibody - ab188594 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue, labeling GOLPH2 with ab188594 at 1/100 dilution followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GOLPH2 antibody - ab97506 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-GOLPH2 antibody (ab97506) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-GOLPH2 antibody - ab88210 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-GOLPH2 antibody (ab88210) at 1 µg/ml + Human colon tissue lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-GOLPH2 antibody - ab22209 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab22209 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22209, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GOLPH2 antibody [6C9] - ab119800 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6C9
Overlay histogram showing HeLa cells stained with ab119800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119800, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-GOLPH2 antibody [EPR3606] - ab109628 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3606
Overlay histogram showing HeLa cells stained with ab109628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109628, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa250-400) - LS-C143567 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
GOLPH2 Antibody - WB detection of GOLM1 in HeLa whole cell extract.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (Internal, clone EPR3606) - LS-C138056 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3606
IHC of paraffin-embedded human colon tissue using anti-GOLPH2 antibody.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa67-81) IHC-plus™ - LS-B2030 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GOLM1 / GOLPH2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2030 dilution 1:100.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (clone 7B3) - LS-C173687 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (clone 3D7) - LS-C173619 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D7
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GOLM1 mouse monoclonal antibody.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa38-87) IHC-plus™ - LS-B5799 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GOLM1 / GOLPH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5799 concentration 5 ug/ml.

Anti-GOLM1 / GP73 / GOLPH2 Antibody (HRP) - LS-C212021 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody - LS-C212022 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody (FITC) - LS-C212024 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody (Biotin) - LS-C212025 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa119-223, Biotin) - LS-C300106 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa133-379, Biotin) - LS-C300107 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GOLM1 / GP73 / GOLPH2 Antibody (aa119-223, FITC) - LS-C302080 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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