Overlay histogram showing HeLa cells stained with ab109628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109628, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilutionLane 1 : LnCaP cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/26344_GOLPH2-Primary-antibodies-ab109628-1.jpg)
All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilutionLane 1 : LnCaP cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution
Immunohistochemical analysis of GOLPH2 in paraffin-embedded Human colon tissue using ab109628 at 1/500 dilution.
ab109628 staining GOLPH2 in Human small intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% milk for 30 min at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody. See Abreview