![All lanes : Anti-GOT2 antibody [3E9] (ab93928) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : PC-12 cell lysateLane 3 : HL-60 cell lysateLane 4 : BCBL-1 cell lysateLane 5 : HepG2 cell lysateLane 6 : NIH/3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/26426_GOT2-Primary-antibodies-ab93928-1.jpg)
All lanes : Anti-GOT2 antibody [3E9] (ab93928) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : PC-12 cell lysateLane 3 : HL-60 cell lysateLane 4 : BCBL-1 cell lysateLane 5 : HepG2 cell lysateLane 6 : NIH/3T3 cell lysate
Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using ab93928 (green) at 100-400 dilution. Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
IHC image of GOT2 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93928, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HepG2 cells stained with ab93928 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93928, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/26429_GOT2-Primary-antibodies-ab93928-6.jpg)
Overlay histogram showing HepG2 cells stained with ab93928 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93928, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.