Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14717
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D9A5G
• Applications: WB IP
• Species Reactivities: Human, Mouse, Rat, Bovine, Dog, Pig
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser93 of human Beclin-1 protein (Ser91 in mouse).
• Description: Rabbit Monoclonal
• Gene: BECN1

Product images

Western blot analysis of extracts from MCF7 and KARPAS-299 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhdrazone (CCCP, 100 μM, 2 hr; +), using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower). Cell line source: Dr Abraham Karpas at the University of Cambridge.

Western blot analysis of extracts from 293T cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 100 μM, 2 hr; +) and mock transfected (-) or transfected with a construct expressing full-length human Beclin-1 (hBeclin-1; +), using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower).

Immunoprecipitation of phospho-Beclin-1 (Ser93) from carbonyl cyanide 3-chlorophenyldydrazone (CCCP)-treated KARPAS-299 cells (100 μM, 2 hr) using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross reactivity with IgG heavy chain. Cell line source: Dr Abraham Karpas at the University of Cambridge.