BECN1

Gene Symbol BECN1
Entrez Gene 8678
Alt Symbol ATG6, VPS30, beclin1
Species Human
Gene Type protein-coding
Description beclin 1, autophagy related
Other Description ATG6 autophagy related 6 homolog|beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)|beclin-1|coiled-coil myosin-like BCL2-interacting protein
Swissprots Q14457 Q9UNA8 O75595 B2R6N7
Accessions EAW60888 EAW60889 EAW60890 Q14457 AF077301 AAC68653 AF139131 AAD27650 AK223411 BAD97131 AK298619 BAG60798 AK299616 BAG61544 AK312651 BAG35534 BC010276 AAH10276 BI496049 BX647788 DC296896 DQ891633 ABM82559 DQ894823 ABM85749 KC776730 AHF22836 L38932 AAB59573 XM_005257758 XP_005257815 XM_005257759 XP_005257816 XM_005257760 XP_005257817 XM_011525421 XP_011523723 NM_003766 NP_003757
Function Plays a central role in autophagy (PubMed:23184933). Required for the abcission step in cytokinesis (PubMed:20208530). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. {ECO:0000269|PubMed:20208530, ECO:0000269|PubMed:23184933, ECO:0000269|PubMed:9765397}.
Subcellular Location Cytoplasm {ECO:0000250|UniProtKB:O88597}. Golgi apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:19050071}; Peripheral membrane protein {ECO:0000269|PubMed:19050071}. Endosome membrane {ECO:0000269|PubMed:23878393}; Peripheral membrane protein {ECO:0000269|PubMed:23878393}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23878393}; Peripheral membrane protein {ECO:0000269|PubMed:23878393}. Mitochondrion membrane {ECO:0000269|PubMed:23878393}; Peripheral membrane protein {ECO:0000269|PubMed:23878393}. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19050071}.
Tissue Specificity Ubiquitous.
Top Pathways Regulation of autophagy

Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb - 14717 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9A5G
Western blot analysis of extracts from MCF7 and KARPAS-299 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhdrazone (CCCP, 100 μM, 2 hr; +), using Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower). Cell line source: Dr Abraham Karpas at the University of Cambridge.

Phospho-Beclin-1 (Ser15) Antibody - 13825 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing full-length human Beclin-1 protein (hBeclin-1; +), mouse Beclin-1 protein (mBeclin-1; +), or mouse ULK1 protein (mULK1; +), using Phospho-Beclin-1 (Ser15) Antibody (upper) and Beclin-1 (D40C5) Rabbit mAb #3495 (lower).

Beclin-1 (D40C5) Rabbit mAb - 3495 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D40C5
Western blot analysis of extracts from various cell lines using Beclin-1 (D40C5) Rabbit mAb.

Beclin-1 Antibody - 3738 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji (human), C6 (rat) and C2C12 (mouse) cell lines, using Beclin-1 Antibody.

Beclin-1 (2A4) Mouse mAb - 4122 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2A4
Western blot analysis of extracts from various cell lines using Beclin-1 (2A4) Mouse mAb.

Phospho-Beclin-1 (Ser93/96) Antibody - 12476 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected or transfected with a construct expressing human Beclin-1 (hBeclin-1), untreated or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 100 μM, 2 hr) as indicated, using Phospho-Beclin-1 (Ser93/96) Antibody (upper) or Beclin-1 (D40C5) Rabbit mAb #3495 (lower).

BECN1 (D-18) - sc-10086 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BECN1 (D-18): sc-10086. Western blot analysis of BECN1 expression in rat prostate tissue extract.

BECN1 (K-15) - sc-10087 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
BECN1 (K-15): sc-10087. Western blot analysis of BECN1 expression in mouse prostate tissue extract.

BECN1 (E-8) - sc-48341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BECN1 (E-8): sc-48341. Western blot analysis of BECN1 expression in non-transfected: sc-110760 (A) and mouse BECN1 transfected: sc-111282 (B) 293 whole cell lysates.

BECN1 (H-300) - sc-11427 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BECN1 (H-300): sc-11427. Western blot analysis of BECN1 expression in MCF7 whole cell lysate.

BECN1 (G-11) - sc-48381 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BECN1 (G-11): sc-48381. Western blot analysis of BECN1 expression in non-transfected: sc-110760 (A) and mouse BECN1 transfected: sc-111282 (B) 293 whole cell lysates.

Anti-Beclin 1 antibody - ab62557 from Abcam

Prices $390.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab62557 staining Beclin 1 in Human MCR5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 21°C. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview

Anti-Beclin 1 antibody - ab62472 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab62472 (1/500) staining Beclin 1 in rat brain tissue sections by IHC-Fr (Immunohistochemistry of frozen sections). Nuclei were counterstained with DAPI.

Anti-Beclin 1 antibody - ab109631 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-Beclin 1 antibody (ab109631) at 0.5 µg/mlLane 2 : Anti-Beclin 1 antibody (ab109631) at 1 µg/mlLane 3 : Anti-Beclin 1 antibody (ab109631) at 2 µg/mlLane 1 : A431 cell lysateLane 2 : A431 cell lysateLane 3 : A431 cell lysate

Anti-Beclin 1 antibody - ab92389 from Abcam

Prices $376.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Beclin 1 antibody (ab92389)Lane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-Beclin 1 antibody [2A4] - ab114071 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A4
All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilutionLane 1 : Hela cell lysateLane 2 : A431 cell lysateLane 3 : MCF7 cell lysateLane 4 : Raji cell lysateLane 5 : Jurkat cell lysateLane 6 : SKBR3 cell lysate

Anti-Beclin 1 antibody [4A10] - ab118148 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A10
All lanes : Anti-Beclin 1 antibody [4A10] (ab118148) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY Beclin 1 Lysates/proteins at 5 µg per lane.

Anti-Beclin 1 antibody [EPR1733Y] - ab51031 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR1733Y
Anti-Beclin 1 antibody [EPR1733Y] (ab51031) at 1/5000 dilution + A431 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled. at 1/2000 dilution

Anti-Beclin 1 antibody [EPR1733Y] (Alexa Fluor® 488) - ab196438 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR1733Y
Overlay histogram showing HeLa cells stained with ab196438 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196438, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Beclin 1 antibody [EPR1733Y] (Alexa Fluor® 647) - ab196188 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR1733Y
Overlay histogram showing HeLa cells stained with ab196188 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196188, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Anti-Beclin 1 antibody [EPR1733Y] (HRP) - ab195792 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR1733Y
Anti-Beclin 1 antibody [EPR1733Y] (HRP) (ab195792) at 1/10000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Beclin 1 antibody - Aminoterminal end - ab55877 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BECN1 / Beclin-1 Antibody (C-Terminus) - LS-C20909 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BECN1 / Beclin-1 Antibody (N-Terminus) - LS-C20910 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-BECN1 / Beclin-1 Antibody - LS-C48605 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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