Anti-GPCR GPR49 antibody

• Supplier: Abcam
• Catalog: ab75732
• Prices: $401.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P FC WB ELISA
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to Human GPCR GPR49 aa 689-719 conjugated to Keyhole Limpet Haemocyanin (KLH)
• Description: Rabbit Polyclonal
• Gene: LGR5
• Conjugate: Unconjugated

Product images

Anti-GPCR GPR49 antibody (ab75732) at 1/750 dilution (for 16 hours at 4°C) + Whole tissue lysate of mouse brain at 18 µgSecondaryAn HRP-conjugated Goat polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GPCR GPR49 antibody (ab75732) at 1/1000 dilution + Mouse cerebellum tissue lysate at 35 µgSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution

ab75732 at 1/50 dilution staining GPCR GPR49 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

ab75732 at 0.025 mg/ml staining GPCR GPR49 in human Hela cells by Immunocytochemistry / Immunofluorescence.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GPCR GPR49 with ab75732. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and then incubated with the primary antibody (1/100, 2 h at room temperature). An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Overlay histogram showing mouse intestinal cells stained with ab75732 (pink line). Cells were incubated with the antibody at 1/50 in DMEM at 20°C for 30 minutes.The secondary antibody used was DyLight® 649 goat anti-rabbit IgG at 1/100 dilution. A non-specific IgG antibody was used for an isotype control (black line).See Abreview

Flow cytometry analysis of HepG2 cells labelling GPCR GRR49 (green) with ab75732 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product References

Combined inhibition of morphogen pathways demonstrates additive antifibrotic - Combined inhibition of morphogen pathways demonstrates additive antifibrotic

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Knockdown of LGR5 suppresses the proliferation of glioma cells in vitro and in - Knockdown of LGR5 suppresses the proliferation of glioma cells in vitro and in

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Distinctive genetic activity pattern of the human dental pulp between deciduous - Distinctive genetic activity pattern of the human dental pulp between deciduous

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The spatial distribution of LGR5+ cells correlates with gastric cancer - The spatial distribution of LGR5+ cells correlates with gastric cancer

Simon E, Petke D, Boger C, Behrens HM, Warneke V, Ebert M, Rocken C. PLoS One. 2012;7(4):e35486.

Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to - Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to

Mardaryev AN, Meier N, Poterlowicz K, Sharov AA, Sharova TY, Ahmed MI, Rapisarda V, Lewis C, Fessing MY, Ruenger TM, Bhawan J, Werner S, Paus R, Botchkarev VA. Development. 2011 Nov;138(22):4843-52.

Expression of Lgr5 in human colorectal carcinogenesis and its potential - Expression of Lgr5 in human colorectal carcinogenesis and its potential

Fan XS, Wu HY, Yu HP, Zhou Q, Zhang YF, Huang Q. Int J Colorectal Dis. 2010 May;25(5):583-90.