LGR5

Gene Symbol LGR5
Entrez Gene 8549
Alt Symbol FEX, GPR49, GPR67, GRP49, HG38
Species Human
Gene Type protein-coding
Description leucine-rich repeat containing G protein-coupled receptor 5
Other Description G-protein coupled receptor 49|G-protein coupled receptor 67|G-protein coupled receptor HG38|leucine-rich repeat-containing G protein-coupled receptor 5|leucine-rich repeat-containing G-protein coupled receptor 5|orphan G protein-coupled receptor HG38
Swissprots Q4VAM2 O75473 Q9UP75 Q4VAM0 D8MCT0
Accessions CDI30209 CDI30210 CDI30211 CDI30212 EAW97258 EAW97259 O75473 AB105190 BAQ19758 AF061444 AAC77911 AF062006 AAC28019 AK075399 AK294985 BAG58053 AK315133 BC096324 AAH96324 BC096325 AAH96325 BC096326 AAH96326 BC099650 AAH99650 BQ774570 CA412916 DA811892 DC323352 DR003044 FN820440 CBL95002 H88004 XM_005269204 XP_005269261 NM_001277226 NP_001264155 NM_001277227 NP_001264156 NM_003667 NP_003658 NR_110596
Function Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. {ECO:0000269|PubMed:21693646, ECO:0000269|PubMed:21727895, ECO:0000269|PubMed:21909076, ECO:0000269|PubMed:22815884, ECO:0000269|PubMed:23809763}.
Subcellular Location Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven (PubMed:23439653). {ECO:0000269|PubMed:23439653}.
Tissue Specificity Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. {ECO:0000269|PubMed:12601349, ECO:0000269|PubMed:16575208, ECO:0000269|PubMed:19030762}.

LGR5 (C-16) - sc-68580 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LGR5 (C-16): sc-68580. Immunofluorescence staining of methanol-fixed HeLa cells showing membrame localization.

LGR5 (H-76) - sc-135238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-GPCR GPR49 antibody - ab75732 from Abcam

Prices $401.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GPCR GPR49 antibody (ab75732) at 1/750 dilution (for 16 hours at 4°C) + Whole tissue lysate of mouse brain at 18 µgSecondaryAn HRP-conjugated Goat polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GPCR GPR49 antibody - ab137484 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Paraffin-embedded sections) analysis of human intestine labelling GPCR GPR49 with ab137484 at 1/100.

Anti-GPCR GPR49 antibody - ab71225 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GPCR GPR49 antibody (ab71225) at 1/1000 dilution + K562 cell line lysates at 35 µgSecondaryHRP goat-anti rabbit (H+L) at 1/5000 dilution

Anti-GPCR GPR49 antibody - ab119324 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GPCR GPR49 antibody (ab119324) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : 3T3 cell lysateLane 3 : HepG2 cell lysate

Anti-GPCR GPR49 antibody - ab128242 from Abcam

Prices $372.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPCR GPR49 antibody [8C9] - ab119012 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8C9
All lanes : Anti-GPCR GPR49 antibody [8C9] (ab119012) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY GPCR GPR49 cDNALysates/proteins at 5 µg per lane.

Anti-GPCR GPR49 antibody [EPR3065Y] - ab75850 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3065Y
Overlay histogram showing SH-SY5Y cells stained with ab75850 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75850, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-GPCR GPR49 antibody - Loop 1 - ab76658 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of GPCR GPR49 in formalin-fixed, paraffin-embedded human brain tissue using ab76658 at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.

Anti-GPR49 / LGR5 Antibody (aa250-550, clone 2H7) - LS-C337374 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H7
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Anti-GPR49 / LGR5 Antibody - LS-C153478 from LifeSpan Bioscience

Prices $305.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (Internal, clone EPR3065Y) - LS-C105455 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3065Y
Western blot analysis on fetal skeletal muscle lysate using anti-GPR49 antibody (LS-C105455), dilution 1:1000.

Anti-GPR49 / LGR5 Antibody (Biotin) - LS-C182008 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (DY650) - LS-C182009 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (aa689-719) - LS-C165757 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
LGR5/GPR49 Antibody western blot of K562 cell line lysates (35 ug/lane). The LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Anti-GPR49 / LGR5 Antibody (aa443-473) - LS-C165758 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
LGR5/GPR49 Antibody western blot of K562 cell line lysates (35 ug/lane). The LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Anti-GPR49 / LGR5 Antibody (DY550) - LS-C182011 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (DY488) - LS-C182010 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (Cytoplasmic Domain) - LS-C190104 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GPR49 / LGR5 Antibody (aa490-494) - LS-C205227 from LifeSpan Bioscience

Prices $265.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa, 3T3 and HepG2 cells using LGR5 (GPR49) antibody.

Anti-GPR49 / LGR5 Antibody (aa250-550, clone 3E11) - LS-C337378 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E11
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-GPR49 / LGR5 Antibody (aa250-550, clone 8C9) - LS-C337376 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8C9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-GPR49 / LGR5 Antibody (aa250-550, clone 14E1) - LS-C337418 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 14E1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-GPR49 / LGR5 Antibody (C-Terminus) - LS-C340545 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyclonal anti-LGR5 antibody at 1:500 dilution.
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