Anti-GPCR LGR6 antibody [EPR6874]
| Name | Anti-GPCR LGR6 antibody [EPR6874] |
|---|---|
| Supplier | Abcam |
| Catalog | ab126747 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR6874 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in the intracellular domain of Human GPCR LGR6 |
| Description | Rabbit Monoclonal |
| Gene | LGR6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilutionLane 1 : Human uterus lysateLane 2 : Human ovary lysateLane 3 : HUVEC lysateLane 4 : Human adrenal gland lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/26928_GPCR-LGR6-Primary-antibodies-ab126747-1.jpg)
All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilutionLane 1 : Human uterus lysateLane 2 : Human ovary lysateLane 3 : HUVEC lysateLane 4 : Human adrenal gland lysateLane 5 : HeLa lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Overlay histogram showing LoVo cells stained with ab126747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126747, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.