Anti-GPCR TGR5 antibody
| Name | Anti-GPCR TGR5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72608 |
| Prices | $382.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to a region between residues 250 and 300 of human GPCR TGR5 (NP_733800 |
| Description | Rabbit Polyclonal |
| Gene | GPBAR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Lane 2 : Anti-GPCR TGR5 antibody (ab72608) at 1/1000 dilutionLanes 3 - 4 : Anti-GPCR TGR5 antibody (ab72608) at 1/5000 dilutionLane 1 : Pre-immune serumLane 2 : Human spleen lysateLane 3 : Mouse spleen lysateLane 4 : Rat spleen lysate
IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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