Anti-Granzyme B antibody
| Name | Anti-Granzyme B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53097 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Polyclonal |
| Gene | GZMB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab53097 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.ab53097 (1/50) staining Granzyme B in paraffin-embbeded human breast carcinoma tissue. Right-hand panel represents a negative control for which ab53097 was pre-incubated with the immunizing peptide.
All lanes : Anti-Granzyme B antibody (ab53097) at 1/300 dilutionLane 1 : NIH/3T3 cell extractLane 2 : NIH/3T3 cell extract with immunizing peptide
Product References
The functionalized human serine protease granzyme B/VEGF(1)(2)(1) targets tumor - The functionalized human serine protease granzyme B/VEGF(1)(2)(1) targets tumor
Mohamedali KA, Cao Y, Cheung LH, Hittelman WN, Rosenblum MG. Mol Cancer Ther. 2013 Oct;12(10):2055-66.
Granzyme-b is involved in mediating post-ischemic neuronal death during focal - Granzyme-b is involved in mediating post-ischemic neuronal death during focal
Chaitanya GV, Schwaninger M, Alexander JS, Babu PP. Neuroscience. 2010 Feb 17;165(4):1203-16. doi: