Anti-Granzyme B antibody - C-terminal (ab175509) at 1/1000 dilution + HL60 cell line lysate at 35 µg
Immunohistochemical analysis of paraffin-embedded Human lymph tissue labeling Granzyme B with ab175509 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometric analysis of HL60 cells labeling Granzyme B with ab175509 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-Granzyme B antibody - C-terminal (ab175509) at 1/1000 dilutionLane 1 : 293 cell lysate, nontransfectedLane 2 : 293 cell lysate, transiently transfected with Granzyme BLysates/proteins at 2 µg per lane.